The global western blotting market is expected to attain a valuation of USD 980.1 million in 2023 and is projected to reach USD 1753.4 million by 2033, trailing a CAGR of 6% during the forecast period.
The market of western blotting is being propelled by several factors such as the emergence of new proteomics applications and a rise in the rates of HIV infection. The prevalence of chronic diseases and growing expenditure on healthcare research activities are also anticipated to fuel demand for western blotting.
The demand for western blotting is further driven by several significant factors, such as increasing preference for personalized medicine and a growing emphasis on maintaining a healthy lifestyle. In addition, the introduction of advanced diagnostic technologies and a heightened awareness of diseases contribute to the overall expansion of the market. The cost-effective and rapid diagnostic capabilities offered by western blotting systems are further expected to fuel the demand for this product.
Report Attribute | Details |
---|---|
Expected Market Value (2023) | US$ 980.1 million |
Anticipated Forecast Value (2033) | US$ 1753.4 million |
Projected Growth Rate (2023 to 2033) | CAGR 6 % |
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The western blotting market was worth USD 695.7 million in 2017. It accumulated a market value of USD 924.3 million in 2022 while growing at a CAGR of 5.8% during the historical period.
The growth of western blotting manufacturers has been fueled by the consistent demand from pharmaceutical and biotechnology companies. Additionally, the expansion of research and development activities in the field of proteomics has served as an additional catalyst for the growth of industrial players in this market.
Protein research dynamics, increased investments in proteomics by biotech and pharmaceutical companies, and the rise in acquired immune deficiency syndrome (AIDS) prevalence are projected to drive the growth of the industry in the forthcoming period.
The precise analysis of protein expression in situ has transformative potential, reducing time to results and increasing throughput, thereby creating growth opportunities in the global market. The transition from traditional film-based exposure to digital imaging systems is anticipated to offer lucrative prospects for both established companies and new entrants. Furthermore, the higher selectivity and sensitivity of the western blot test, enabling precise quantification with smaller sample volumes, is expected to drive its increased application and future market growth.
The rise in research activities and increased research and development spending by pharmaceutical and biotechnology companies are further expected to create lucrative opportunities for market expansion.
The market is projected to be worth USD 1753.4 million by the end of forecast period while exhibiting a CAGR of 6%.
Use of Western Blotting to Detect Infectious Agents to Drive Market Growth
The demand for western blotting is expected to witness substantial growth due to the increasing application of the western blot test, capable of detecting protein at picogram levels. This technology finds utility in various applications, including early diagnosis, as it allows the identification of immunogenic reactions from infectious agents such as viruses and bacteria. The technique, widely used in HIV detection, utilizes both antigens and antisera, offering high specificity independent of antibody specificity. These factors drive its expanded application and contribute to the market's revenue growth.
Western blotting products, including traditional wet transfer blotting systems, chromogenic reagent kits, and more, play a vital role in scientific research, particularly in the biotechnology sector. Consequently, increased investments and related activities in this sector are anticipated to propel market growth during the forecast period.
Lack of Specialized Expertise to Restrain Market Expansion
High costs of tagged antibodies and laboratory equipment, as well as the requirement for specialized expertise to perform the test, are expected to restrain market expansion. Additionally, the time-consuming nature of western blotting compared to enzyme-linked immunosorbent assay (ELISA) and its susceptibility to inaccurate or subjective results further impede market revenue growth.
North America Driving Growth with Significant CAGR
The North American western blotting market accounted for a revenue share of 32% in 2022. The United States alone accounted for a revenue share of 29.6% in the same year. The demand outlook for western blotting in the region is expected to grow with a CAGR of 5.8% during the forecast period.
The region is expected to dominate the market of western blotting due to rising prevalence of Human Immune Deficiency (HIV) and Lyme disease across this region.
The presence of an advanced healthcare system, growing investments in proteomics research, and an increasing focus on personalized therapy and genetically engineered crops are key factors influencing the regional landscape.
The growth of the studied market in North America is expected to be driven by the increasing prevalence of chronic diseases and the rising research and development investments by pharmaceutical and biotech companies. According to the 2022 update from the Centre for Disease Control (CDC), six in ten Americans live with at least one chronic disease, such as heart disease, stroke, cancer, or diabetes, which are the leading causes of death and disability in the region. To enhance the quality of life and mitigate risks, early diagnosis of chronic diseases is crucial, and western blotting is expected to play a significant role in this regard, thereby contributing to market growth.
Throughout the forecast period, the United States is expected to sustain its leadership position as the leading region in the North American market. The market in this region is expected to grow with a CAGR of 5.9% throughout the forecast period.
The presence of highly advanced biotechnology and medical diagnostics sectors is expected to drive the sales of western blotting products in the years to come. For example, as of October 2022, the United States Department of Health & Human Services reported that around 1.2 million individuals in the country are living with HIV, with approximately 13% of them unaware of their HIV status and in need of testing.
China Driving Growth with a Market Share above 15%
The western blotting market in China is expected to grow with a significant CAGR of 7.4% during the forecast period. The region is expected to dominate the Asia Pacific market by accumulating a 19.8% market share in 2023.
China has emerged as a highly progressive market in the rapidly growing western blotting market of Asia-Pacific. The exponential growth of the pharmaceutical industry and increasing demand from the medical diagnostics sector are driving the expansion of western blotting suppliers in China.
Rising population, booming healthcare sector, and technological advancements in the region are major factors driving industry growth.
Technological advancements in western blotting techniques, including enhanced imaging systems, automated equipment, and improved detection methods, have exerted a notable influence on the regional market. These advancements have enhanced the sensitivity, accuracy, and speed of western blotting assays, thereby driving growth in the region.
The growing emphasis on personalized medicine and targeted therapies has led to an increased demand for the identification and validation of biomarkers. This is anticipated to spur growth in the market of China.
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Reagent Kits to Drive Growth with Significant CAGR
Reagent kits dominated the western blotting market by accounting for 58.81% of revenue share in 2022. The segment is expected to grow with a significant CAGR of 7.2% throughout the forecast period.
The high share and growth of these reagent consumables can primarily be attributed to their extensive use and massive consumption during the western blotting process.
Ongoing advancements in research activities, particularly in the fields of proteomics, genomics, and molecular biology, are expected to fuel the demand for reagent kits in western blotting. These kits allow for efficient and accurate protein analysis, aiding in the discovery and validation of biomarkers.
Scientific Research Propelling Growth with a Promising CAGR
In 2022, scientific research accounted for a sizeable share of 38.20% in the market of western blotting. The segment is expected to flourish at a CAGR of 7% during the forecast period.
Scientific research is currently a profitable segment in the global western blotting industry, and it is expected to maintain its position in the years ahead. The increasing number of research and development activities and advancements in the medical diagnostics sector are attractive heavy investments from key players, thereby fuelling segment growth.
Academic and research institutes extensively employ western blotting as an effective method to determine protein presence, relative abundance, relative mass, Post-Translational Modification (PTM), and protein-protein interactions. Western blotting enables researchers to identify specific proteins within complex mixtures isolated from cells. The growth of this segment is driven by increasing government spending in research activities and a surge in proteomics research endeavors.
Start-ups play a crucial role in identifying growth opportunities in various markets, including the western blotting market. Their ability to generate high returns contributes to the expansion of the industry. Start-ups are often more efficient in converting inputs into outputs and are adaptable to unpredictable market conditions. Here are some start-ups expected to drive growth in the western blotting market.
The western blotting market is extremely competitive and consists of various key industry players. These players are heavily investing in manufacturing western blotting.
The key industry players are Thermo Fisher Scientific, Inc., GE Healthcare, Hofmann-La Roche Ltd., Danaher Corporation (Lumigen, Inc.), EMD Millipore Corporation, Bio-Rad Laboratories, Inc., PerkinElmer Inc., LI-COR, Inc., Bio-Techne Corporation (ProteinSimple Inc.), Advansta, Inc.
Some recent developments in the market are:
Key industry players leverage organic growth strategies like acquisition, mergers, partnerships, and collaboration to enhance their product portfolio. This is expected to fuel the global western blotting market.
Report Attribute | Details |
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Market Value in 2023 | US$ 980.1 million |
Market Value in 2033 | US$ 1753.4 million |
Growth Rate | CAGR of 6% from 2023 to 2033 |
Base Year for Estimation | 2022 |
Historical Data | 2017 to 2022 |
Forecast Period | 2023 to 2033 |
Quantitative Units | Revenue in USD million and CAGR from 2023 to 2033 |
Report Coverage | Revenue Forecast, Volume Forecast, Company Ranking, Competitive Landscape, Growth Factors, Trends, and Pricing Analysis |
Segments Covered |
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Regions Covered |
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Key Countries Profiled |
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Key Companies Profiled |
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Customization | Available Upon Request |
The projected revenue of the market by 2033 is US$ 1,753.4 million.
The market CAGR for 2033 is projected to be 6%.
North America, Europe, and Asia-Pacific are the leading market.
North America holds the largest share of the Western Blotting market.
North America is growing fast due to the high research and development activities.
1. Executive Summary
1.1. Global Market Outlook
1.2. Demand-side Trends
1.3. Supply-side Trends
1.4. Technology Roadmap Analysis
1.5. Analysis and Recommendations
2. Market Overview
2.1. Market Coverage / Taxonomy
2.2. Market Definition / Scope / Limitations
3. Market Background
3.1. Market Dynamics
3.1.1. Drivers
3.1.2. Restraints
3.1.3. Opportunity
3.1.4. Trends
3.2. Scenario Forecast
3.2.1. Demand in Optimistic Scenario
3.2.2. Demand in Likely Scenario
3.2.3. Demand in Conservative Scenario
3.3. Opportunity Map Analysis
3.4. Investment Feasibility Matrix
3.5. PESTLE and Porter’s Analysis
3.6. Regulatory Landscape
3.6.1. By Key Regions
3.6.2. By Key Countries
3.7. Regional Parent Market Outlook
4. Global Market Analysis 2018 to 2022 and Forecast, 2023 to 2033
4.1. Historical Market Size Value (US$ Million) Analysis, 2018 to 2022
4.2. Current and Future Market Size Value (US$ Million) Projections, 2023 to 2033
4.2.1. Y-o-Y Growth Trend Analysis
4.2.2. Absolute $ Opportunity Analysis
5. Global Market Analysis 2018 to 2022 and Forecast 2023 to 2033, By Product
5.1. Introduction / Key Findings
5.2. Historical Market Size Value (US$ Million) Analysis By Product, 2018 to 2022
5.3. Current and Future Market Size Value (US$ Million) Analysis and Forecast By Product, 2023 to 2033
5.3.1. Electrophoresis Blotting Systems
5.3.1.1. Automated Dry Blotting Systems
5.3.1.2. Semi-Dry Blotting Systems
5.3.1.3. Traditional Wet Transfer Blotting Systems
5.3.2. Reagent Kits
5.3.2.1. Chromogenic Reagent Kits
5.3.2.2. Chemiluminescent Reagent Kits
5.3.2.3. Fluorescent Reagents
5.3.2.4. Antibodies
5.3.2.5. Buffer Kits
5.4. Y-o-Y Growth Trend Analysis By Product, 2018 to 2022
5.5. Absolute $ Opportunity Analysis By Product, 2023 to 2033
6. Global Market Analysis 2018 to 2022 and Forecast 2023 to 2033, By Application
6.1. Introduction / Key Findings
6.2. Historical Market Size Value (US$ Million) Analysis By Application, 2018 to 2022
6.3. Current and Future Market Size Value (US$ Million) Analysis and Forecast By Application, 2023 to 2033
6.3.1. Research
6.3.2. Diagnostics
6.3.3. Agricultural
6.3.4. Food & Beverages
6.3.5. Other
6.4. Y-o-Y Growth Trend Analysis By Application, 2018 to 2022
6.5. Absolute $ Opportunity Analysis By Application, 2023 to 2033
7. Global Market Analysis 2018 to 2022 and Forecast 2023 to 2033, By End User
7.1. Introduction / Key Findings
7.2. Historical Market Size Value (US$ Million) Analysis By End User, 2018 to 2022
7.3. Current and Future Market Size Value (US$ Million) Analysis and Forecast By End User, 2023 to 2033
7.3.1. Diagnostic Laboratories
7.3.2. Research Institutions
7.3.3. Pharmaceutical & Biotechnology Companies
7.4. Y-o-Y Growth Trend Analysis By End User, 2018 to 2022
7.5. Absolute $ Opportunity Analysis By End User, 2023 to 2033
8. Global Market Analysis 2018 to 2022 and Forecast 2023 to 2033, By Region
8.1. Introduction
8.2. Historical Market Size Value (US$ Million) Analysis By Region, 2018 to 2022
8.3. Current Market Size Value (US$ Million) Analysis and Forecast By Region, 2023 to 2033
8.3.1. North America
8.3.2. Latin America
8.3.3. Europe
8.3.4. South Asia
8.3.5. East Asia
8.3.6. Oceania
8.3.7. MEA
8.4. Market Attractiveness Analysis By Region
9. North America Market Analysis 2018 to 2022 and Forecast 2023 to 2033, By Country
9.1. Historical Market Size Value (US$ Million) Trend Analysis By Market Taxonomy, 2018 to 2022
9.2. Market Size Value (US$ Million) Forecast By Market Taxonomy, 2023 to 2033
9.2.1. By Country
9.2.1.1. USA
9.2.1.2. Canada
9.2.2. By Product
9.2.3. By Application
9.2.4. By End User
9.3. Market Attractiveness Analysis
9.3.1. By Country
9.3.2. By Product
9.3.3. By Application
9.3.4. By End User
9.4. Key Takeaways
10. Latin America Market Analysis 2018 to 2022 and Forecast 2023 to 2033, By Country
10.1. Historical Market Size Value (US$ Million) Trend Analysis By Market Taxonomy, 2018 to 2022
10.2. Market Size Value (US$ Million) Forecast By Market Taxonomy, 2023 to 2033
10.2.1. By Country
10.2.1.1. Brazil
10.2.1.2. Mexico
10.2.1.3. Rest of Latin America
10.2.2. By Product
10.2.3. By Application
10.2.4. By End User
10.3. Market Attractiveness Analysis
10.3.1. By Country
10.3.2. By Product
10.3.3. By Application
10.3.4. By End User
10.4. Key Takeaways
11. Europe Market Analysis 2018 to 2022 and Forecast 2023 to 2033, By Country
11.1. Historical Market Size Value (US$ Million) Trend Analysis By Market Taxonomy, 2018 to 2022
11.2. Market Size Value (US$ Million) Forecast By Market Taxonomy, 2023 to 2033
11.2.1. By Country
11.2.1.1. Germany
11.2.1.2. UK
11.2.1.3. France
11.2.1.4. Spain
11.2.1.5. Italy
11.2.1.6. Rest of Europe
11.2.2. By Product
11.2.3. By Application
11.2.4. By End User
11.3. Market Attractiveness Analysis
11.3.1. By Country
11.3.2. By Product
11.3.3. By Application
11.3.4. By End User
11.4. Key Takeaways
12. South Asia Market Analysis 2018 to 2022 and Forecast 2023 to 2033, By Country
12.1. Historical Market Size Value (US$ Million) Trend Analysis By Market Taxonomy, 2018 to 2022
12.2. Market Size Value (US$ Million) Forecast By Market Taxonomy, 2023 to 2033
12.2.1. By Country
12.2.1.1. India
12.2.1.2. Malaysia
12.2.1.3. Singapore
12.2.1.4. Thailand
12.2.1.5. Rest of South Asia
12.2.2. By Product
12.2.3. By Application
12.2.4. By End User
12.3. Market Attractiveness Analysis
12.3.1. By Country
12.3.2. By Product
12.3.3. By Application
12.3.4. By End User
12.4. Key Takeaways
13. East Asia Market Analysis 2018 to 2022 and Forecast 2023 to 2033, By Country
13.1. Historical Market Size Value (US$ Million) Trend Analysis By Market Taxonomy, 2018 to 2022
13.2. Market Size Value (US$ Million) Forecast By Market Taxonomy, 2023 to 2033
13.2.1. By Country
13.2.1.1. China
13.2.1.2. Japan
13.2.1.3. South Korea
13.2.2. By Product
13.2.3. By Application
13.2.4. By End User
13.3. Market Attractiveness Analysis
13.3.1. By Country
13.3.2. By Product
13.3.3. By Application
13.3.4. By End User
13.4. Key Takeaways
14. Oceania Market Analysis 2018 to 2022 and Forecast 2023 to 2033, By Country
14.1. Historical Market Size Value (US$ Million) Trend Analysis By Market Taxonomy, 2018 to 2022
14.2. Market Size Value (US$ Million) Forecast By Market Taxonomy, 2023 to 2033
14.2.1. By Country
14.2.1.1. Australia
14.2.1.2. New Zealand
14.2.2. By Product
14.2.3. By Application
14.2.4. By End User
14.3. Market Attractiveness Analysis
14.3.1. By Country
14.3.2. By Product
14.3.3. By Application
14.3.4. By End User
14.4. Key Takeaways
15. MEA Market Analysis 2018 to 2022 and Forecast 2023 to 2033, By Country
15.1. Historical Market Size Value (US$ Million) Trend Analysis By Market Taxonomy, 2018 to 2022
15.2. Market Size Value (US$ Million) Forecast By Market Taxonomy, 2023 to 2033
15.2.1. By Country
15.2.1.1. GCC Countries
15.2.1.2. South Africa
15.2.1.3. Israel
15.2.1.4. Rest of MEA
15.2.2. By Product
15.2.3. By Application
15.2.4. By End User
15.3. Market Attractiveness Analysis
15.3.1. By Country
15.3.2. By Product
15.3.3. By Application
15.3.4. By End User
15.4. Key Takeaways
16. Key Countries Market Analysis
16.1. USA
16.1.1. Pricing Analysis
16.1.2. Market Share Analysis, 2022
16.1.2.1. By Product
16.1.2.2. By Application
16.1.2.3. By End User
16.2. Canada
16.2.1. Pricing Analysis
16.2.2. Market Share Analysis, 2022
16.2.2.1. By Product
16.2.2.2. By Application
16.2.2.3. By End User
16.3. Brazil
16.3.1. Pricing Analysis
16.3.2. Market Share Analysis, 2022
16.3.2.1. By Product
16.3.2.2. By Application
16.3.2.3. By End User
16.4. Mexico
16.4.1. Pricing Analysis
16.4.2. Market Share Analysis, 2022
16.4.2.1. By Product
16.4.2.2. By Application
16.4.2.3. By End User
16.5. Germany
16.5.1. Pricing Analysis
16.5.2. Market Share Analysis, 2022
16.5.2.1. By Product
16.5.2.2. By Application
16.5.2.3. By End User
16.6. UK
16.6.1. Pricing Analysis
16.6.2. Market Share Analysis, 2022
16.6.2.1. By Product
16.6.2.2. By Application
16.6.2.3. By End User
16.7. France
16.7.1. Pricing Analysis
16.7.2. Market Share Analysis, 2022
16.7.2.1. By Product
16.7.2.2. By Application
16.7.2.3. By End User
16.8. Spain
16.8.1. Pricing Analysis
16.8.2. Market Share Analysis, 2022
16.8.2.1. By Product
16.8.2.2. By Application
16.8.2.3. By End User
16.9. Italy
16.9.1. Pricing Analysis
16.9.2. Market Share Analysis, 2022
16.9.2.1. By Product
16.9.2.2. By Application
16.9.2.3. By End User
16.10. India
16.10.1. Pricing Analysis
16.10.2. Market Share Analysis, 2022
16.10.2.1. By Product
16.10.2.2. By Application
16.10.2.3. By End User
16.11. Malaysia
16.11.1. Pricing Analysis
16.11.2. Market Share Analysis, 2022
16.11.2.1. By Product
16.11.2.2. By Application
16.11.2.3. By End User
16.12. Singapore
16.12.1. Pricing Analysis
16.12.2. Market Share Analysis, 2022
16.12.2.1. By Product
16.12.2.2. By Application
16.12.2.3. By End User
16.13. Thailand
16.13.1. Pricing Analysis
16.13.2. Market Share Analysis, 2022
16.13.2.1. By Product
16.13.2.2. By Application
16.13.2.3. By End User
16.14. China
16.14.1. Pricing Analysis
16.14.2. Market Share Analysis, 2022
16.14.2.1. By Product
16.14.2.2. By Application
16.14.2.3. By End User
16.15. Japan
16.15.1. Pricing Analysis
16.15.2. Market Share Analysis, 2022
16.15.2.1. By Product
16.15.2.2. By Application
16.15.2.3. By End User
16.16. South Korea
16.16.1. Pricing Analysis
16.16.2. Market Share Analysis, 2022
16.16.2.1. By Product
16.16.2.2. By Application
16.16.2.3. By End User
16.17. Australia
16.17.1. Pricing Analysis
16.17.2. Market Share Analysis, 2022
16.17.2.1. By Product
16.17.2.2. By Application
16.17.2.3. By End User
16.18. New Zealand
16.18.1. Pricing Analysis
16.18.2. Market Share Analysis, 2022
16.18.2.1. By Product
16.18.2.2. By Application
16.18.2.3. By End User
16.19. GCC Countries
16.19.1. Pricing Analysis
16.19.2. Market Share Analysis, 2022
16.19.2.1. By Product
16.19.2.2. By Application
16.19.2.3. By End User
16.20. South Africa
16.20.1. Pricing Analysis
16.20.2. Market Share Analysis, 2022
16.20.2.1. By Product
16.20.2.2. By Application
16.20.2.3. By End User
16.21. Israel
16.21.1. Pricing Analysis
16.21.2. Market Share Analysis, 2022
16.21.2.1. By Product
16.21.2.2. By Application
16.21.2.3. By End User
17. Market Structure Analysis
17.1. Competition Dashboard
17.2. Competition Benchmarking
17.3. Market Share Analysis of Top Players
17.3.1. By Regional
17.3.2. By Product
17.3.3. By Application
17.3.4. By End User
18. Competition Analysis
18.1. Competition Deep Dive
18.1.1. Thermo Fisher Scientific, Inc.
18.1.1.1. Overview
18.1.1.2. Product Portfolio
18.1.1.3. Profitability by Market Segments
18.1.1.4. Sales Footprint
18.1.1.5. Strategy Overview
18.1.1.5.1. Marketing Strategy
18.1.2. GE Healthcare
18.1.2.1. Overview
18.1.2.2. Product Portfolio
18.1.2.3. Profitability by Market Segments
18.1.2.4. Sales Footprint
18.1.2.5. Strategy Overview
18.1.2.5.1. Marketing Strategy
18.1.3. Hofmann-La Roche Ltd.
18.1.3.1. Overview
18.1.3.2. Product Portfolio
18.1.3.3. Profitability by Market Segments
18.1.3.4. Sales Footprint
18.1.3.5. Strategy Overview
18.1.3.5.1. Marketing Strategy
18.1.4. Danaher Corporation (Lumigen, Inc.)
18.1.4.1. Overview
18.1.4.2. Product Portfolio
18.1.4.3. Profitability by Market Segments
18.1.4.4. Sales Footprint
18.1.4.5. Strategy Overview
18.1.4.5.1. Marketing Strategy
18.1.5. EMD Millipore Corporation
18.1.5.1. Overview
18.1.5.2. Product Portfolio
18.1.5.3. Profitability by Market Segments
18.1.5.4. Sales Footprint
18.1.5.5. Strategy Overview
18.1.5.5.1. Marketing Strategy
18.1.6. Bio-Rad Laboratories, Inc.
18.1.6.1. Overview
18.1.6.2. Product Portfolio
18.1.6.3. Profitability by Market Segments
18.1.6.4. Sales Footprint
18.1.6.5. Strategy Overview
18.1.6.5.1. Marketing Strategy
18.1.7. PerkinElmer Inc.
18.1.7.1. Overview
18.1.7.2. Product Portfolio
18.1.7.3. Profitability by Market Segments
18.1.7.4. Sales Footprint
18.1.7.5. Strategy Overview
18.1.7.5.1. Marketing Strategy
18.1.8. LI-COR, Inc.
18.1.8.1. Overview
18.1.8.2. Product Portfolio
18.1.8.3. Profitability by Market Segments
18.1.8.4. Sales Footprint
18.1.8.5. Strategy Overview
18.1.8.5.1. Marketing Strategy
18.1.9. Bio-Techne Corporation (ProteinSimple Inc.)
18.1.9.1. Overview
18.1.9.2. Product Portfolio
18.1.9.3. Profitability by Market Segments
18.1.9.4. Sales Footprint
18.1.9.5. Strategy Overview
18.1.9.5.1. Marketing Strategy
18.1.10. Advansta, Inc.
18.1.10.1. Overview
18.1.10.2. Product Portfolio
18.1.10.3. Profitability by Market Segments
18.1.10.4. Sales Footprint
18.1.10.5. Strategy Overview
18.1.10.5.1. Marketing Strategy
19. Assumptions & Acronyms Used
20. Research Methodology
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